Lab Master Help Page

Rights and Responsibilities of the Lab Master

The Lab Master designation is applied to any user who has created a Sesame lab group. In addition to controlling the membership of the group (through both inviting and deleting users), the Lab Master also is responsible for defining the Lab Resources that all group members use when entering data into Sesame. Most of these resources will be used to populate the pull down menus found in various record types (a few of the resources are more complex and will be discussed on the next page). The majority of these lab resources are organized in tables where the Lab Master can added or delete entries. The Lab Master accesses the different resources through the Options pull down menu.

The table below outlines the different types of list-style resources, the views in Sheherazade to which they apply and the type of entries that the Lab Master should make. More complex resources will be dealt with on the following page.

Resource	View	Table Entries
Status	All	List of status options for different record types (ie. active, inactive)
Type	All	List of type options for different record types (ie. R&D, production)
File Type	All	List of all file types (PDF, Word document, etc) that may be appended to a record
Plates	Work Group, Crystal Screen	List of all different plate types which may be used
Source Organism	ORF	List of all organisms for which gene models may be loaded into the database
Bar Code Printers	Crystal Screen	List of all specialized bar code printers
Locations (Room)	All	List of all rooms where a product (plasmid, protein, etc) may be stored
Locations (Freezer)	All	List of all freezers/refrigerators where a product (plasmid, protein, etc) may be stored
Locations (Rack View)	All	List of all racks within a freezer or refrigerator where a product (plasmid, protein, etc) may be stored
Locations (Shelf View)	Crystal Screen	List of all shelves where the plates from a crystallization screen may be stored
5' Prefix	Work Group, ORF	List of all generic sequences which may be added to a primer made to the 5' end of an ORF
3' Prefix	Work Group, ORF	List of all generic sequences which may be added to a primer made to the 3' end of an ORF
Plasmid Components (Plasmid Source)	Plasmid	List of all parent vectors into which an ORF may be cloned
Plasmid Components (Host)	Plasmid	List of all bacterial strains that a plasmid may be transformed into
Plasmid Components (Restriction)	Plasmid, ORF, Work Group	List of all restriction enzymes used during cloning
Plasmid Components (Tag)	Plasmid	List of all purification and/or detection tags that may be fused to a protein
Plasmid Components (Cleavage)	Plasmid	List of all protease cleavage sites that may be engineered into a fusion protein
Lock Solvent	Sample	List of lock solvents that may be included in an NMR sample
Int. Ref. Compound	Sample	List of internal reference compounds that may be included in an NMR sample
Mass Spectrum Type	Mass Sample	List of different types of mass spec experiments (ESI, MALDI, tryptic digestion, etc)
Mass Spectometer	Mass Sample	List of different types of mass spec instrument that may be used
Stock Solutions (Precipitant)	Crystal Screen	List of all precipitants used in a protein crystallization screen
Stock Solutions (Salt)	Crystal Screen	List of all salts used in a protein crystallization screen
Stock Solutions (Buffer)	Crystal Screen	List of all buffers used in a protein crystallization screen
Stock Solutions (Additive)	Crystal Screen	List of all additives used in a protein crystallization screen
Stock Solutions (pH Adjuster)	Crystal Screen	List of all pH adjusters used in a protein crystallization screen
Stock Solutions (Premade Solution)	Crystal Screen	List of all premade solutions used in a protein crystallization screen
Stock Solutions (Buffered Precipitant)	Crystal Screen	List of all buffered precipitants used in a protein crystallization screen
Stock Solutions (Cryoprotectant)	Crystal Screen	List of all cryoprotectants used in a protein crystallization screen
Robot Rack Type View	Crystal Screen	List of all generic rack configurations used by a robot in preparing a protein crystallization screen
Crystalscore View	Crystal Screen	List of all scoring options when using the CrystalScore imaging system

With one exception, the Systems Recources list contains a set of table that apply to all lab groups using Sesame and can only be modified by the Sesame Administrator (and not by individual Lab Masters). For more information on System Resources or requested changes, contact your local Sesame Administrator. The Bulk load option is active for Lab Masters. Using options found in the Bulk Load menu, Lab Masters can add or update scores associated with specific targets or add database references. Scores are attached to ORFs through uploading a comma separated value (CSV) file with the format:

ORF ID, score
GO.1, 1234567890
GO.2, 1345678900
etc

Where the GO.xxx number is the ORF number of the target. Note that the first line of the CSV file should be a header and will be ignored by Sesame. The target score will be unique to every group. For an example of one type of target score visit the Bioinformatics section of the web site for the Center for Eukaryotic Structural Genomics.

Lab Masters can also add database references to specific targets. References are attached to ORFs through uploading a comma separated value (CSV) file with the format:

ORF ID, database, database id
GO.1, PDB, 1RJJ
GO.2, PDB, 1T0G
GO.2, BMRB, 6138
etc

Where the GO.xxx number is the ORF number of the target. Note that the first line of the CSV file should be a header and will be ignored by Sesame. Any number of references can be added to an ORF.

Sesame Overview - Sesame Modules - Viewing Records - Linking Records
Lab Master Info - Sheherazade - Sand - Sundial - Jafar - Rukh - Camel