The Sesame Modules |
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| Sesame is designed to capture and organize data on a variety of different laboratory activities. Although data is stored
in a single database, data access is achieved through several different modules. Furthermore, within a given module, several
different views of the data may be possible. The user can toggle between different views through the
Options menu. Within each view, records can be created on the processes and products of wet lab work. The
specific functionalities in each module and view will be dealt with separately on the subsequent help pages. |
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- Sheherazade Module
- Open Reading Frame View - for organizing and viewing bioinformatics data on predicted ORFs
- Work Group View - for organizing ORFs into functional groups and tracking their progress in the lab
- Primer View - for capturing data on oligo nucleotides
- Plasmid View - for capturing data on plasmid constructs
- Protein View - for capturing data on the production and purification of proteins
- Sample View - for documenting the components of a purified sample
- Mass Sample View - for capturing data on samples sent for mass spec analysis
- Crystal Screen View - for capturing data on protein cyrstallization screens
- Rukh Modules
- Y2H Request View - for requesting a yeast two hybrid screen
- BaitView - for capturing data on yeast two hybrid screen baits
- Y2H Screen View - for capturing data on the implementation of a yeast two hybrid screen
- Y2H Plate Read View - for capturing data on the results of a yeast two hybrid screen
- Sand/Sundial Modules
- Jafar Module
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| This organization allows user to create clear and concise records on a given aspect of the project (such as a plasmid construction,
a protein purification or a sample prepared for data collection). Details on how to link different record types together to create
an electronic papertrail across the whole project will be covered on a subsequent page. |
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Data can be entered into Sesame in a number of ways.
- Text can be entered into predefined fields within a record. For example, records can be given a name and a designation (such R&D or
production scale).
- In some record types, a set of physical parameters can be calculated for a protein sequence entered by a user.
- In all record types, Actions can be added to document specific lab activities. Actions are defined
by the Lab Master and can either represent binary outcomes (such as "PCR successful" or "PCR unsuccessful") or a range of
outcomes (such as "greater than 20mg protein purified", "10-20mg protein purified" or "less than 10mg protein purified").
Note that because actions can only be defined by the Lab Master, the vocabularly is controlled and it is not possible for
users to record unique values using actions.
- Files and images can be uploaded and attached to any record.
- In all record types, Protocol Trees can be defined and filled in to capture data not expressly
asked for elsewhere, including unique experimental values.
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| Images and other file types can be associated with any record type. The files are stored in the Sesame database and are immediately
available to all lab members. All lab members can add, view and download images and files, although a user may only delete an
image or file that they have added. To add an image or file, click the green button on the righthand side of the field. Enter the
necessary information and select the file. To download a file, highlight the appropriate file and click the yellow button and
specify the path (note that you do not have to specify the file name as that is taken from the database). Images can also be
viewed within Sesame. Highlight the image and click the blue button, alternatively, double clicking image will also open the
image. The image will appear on the Board. Many standard image manipulate tools are available in the image window. |
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Once an image or
file has been attached to a record, pointers to that file can be created within other records (as oppossed to uploading the same
file multiple times). Simply highlight the appropriate line in the Files or Images table and drag and drop the line on to the frame
of the other record. |
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