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The Work Group View

The Work Group View in Sesame allows the user to group multiple ORFs together into a single unit known as a Work Group which is then the focus of lab activities. This functionality is exceedingly important in high throughput projects where targets are often worked on in the 96 well format. Users can view existing work groups by searching the lab group records (discussed on the General Principles help page). Alternatively, new work groups can be created using the New functionality in the Options menu of the search window (or in the Window menu of the Board). A blank record will be displayed on the Board, consisting of two panels, the General Panel and the ORF Panel. The General Panel is used to capture basic information about the work group. Read/write permissions vary between fields, some fields are wholly uneditable, some can only be altered by the Owner (or creator) of the record and some can be modified by all lab group members.

Work Group record

While the definitions given below can act as a guide to understanding the usage of fields present in a work group record, many of the field are flexible and can be tailored to the needs of the lab group. The table below can be used as a guide to the use of some of the fields.

FieldPermissionDefinition
Database No.UneditableUnique Sesame identifier
External IDAllReference to a group specific identifier
OwnerUneditableCreator of the record
LabUneditableLab Group
StatusOwnerWork group status (status options are defined by Lab Master)
TypeOwnerWork group category (type options are defined by Lab Master)
WorkGroup NameOwnerWork group identifier
User LabelOwnerAlternate work group identifier
Lab ProtocolOwnerLab method applied to the work group
DateOwnerDate of work group creation (but is editable)
Plate TypeOwnerPlate format
ORFlist LockedOwnerDenotes the status of the list of ORFs
Linked ItemsUneditableOther records related to current record
RoomAllPhysical location of products
FreezerAllPhysical location of products
InfoAllInformation not specifically asked for elsewhere
ImagesAllJPG or GIF images associated with record
FilesAllOther files associated with record
ProtocolAllLab Master defined tree to accept experimental results

The ORF panel contains a list of all of the ORFs contained within the work group. It is important to note that a work group can contain more ORFs than can fit in the chosen plate format. In these instances, the ORFs are split over multiple plates in the same work group. As in the ORF view, the panel displays basic information about the ORFs and further details can be viewed by double clicking a given ORF. This panel can also be customized by reorganizing and resizing columns and by hiding columns using the Show/Hide Columns function in the Edit menu. Specific ORFs can be located in a work group using the functionalities in the Search menu.

A few columns are unique to the Work Group ORF panel. The Well Coord column contains the location of the ORF in the work group. The format of this column is (Work_Group_No.Plate_No.Well_Position). The Prev. Coord column displays the link to the previous work group and position that the ORF was a part of. The information represents part of the electronic paper trail that Sesame maintains. If an ORF was added to the work group from the ORF View (as opposed from an existing work group), this column will be empty. The Selection Score is the target score of the ORF at the time that it was incorporated into the current work group. A list of actions added to each ORF in the current work group can be found at the end of the row.

ORF panel of the work group view

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Open Reading Frame View - Open Reading Frame Details - Work Group View - Work Group Manipulations - Work Group Actions
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